{"id":202147,"date":"2025-10-28T14:08:55","date_gmt":"2025-10-28T17:08:55","guid":{"rendered":"https:\/\/controllab.com\/ensino\/resumos-cientificos\/desempenho-laboratorial-na-deteccao-molecular-de-hpv-de-alto-risco-kits-comerciais-superam-o-desempenho-dos-testes-desenvolvidos-em-laboratorio\/"},"modified":"2025-10-30T11:05:45","modified_gmt":"2025-10-30T14:05:45","slug":"laboratory-performance-in-molecular-detection-of-high-risk-hpv-commercial-kits-outperform-laboratory-developed-tests","status":"publish","type":"resumos","link":"https:\/\/controllab.com\/en\/learning\/scientific-abstracts\/laboratory-performance-in-molecular-detection-of-high-risk-hpv-commercial-kits-outperform-laboratory-developed-tests\/","title":{"rendered":"Laboratory Performance in Molecular Detection of High-Risk HPV: Commercial Kits Outperform Laboratory-Developed Tests"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"202147\" class=\"elementor elementor-202147 elementor-201641\" data-elementor-post-type=\"resumos\">\n\t\t\t\t<div class=\"has_ae_slider elementor-element elementor-element-3f570f4 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"3f570f4\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8e23f20 elementor-widget elementor-widget-heading\" data-id=\"8e23f20\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Background - Aim<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-22ee735 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"22ee735\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c82d634 elementor-widget elementor-widget-text-editor\" data-id=\"c82d634\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p align=\"justify\">High-risk HPV causes cervical cancer, a global health challenge. Molecular DNA testing enables early detection, allowing timely treatment, improving screening, stratifying risks, and optimizing follow-ups. Reliable results require standardized laboratory performance. External Quality Assessment Programs (EQAP) ensure accuracy (AC) and harmonization in HPV diagnostics. This study aimed to evaluate the performance of laboratories in an EQAP for high-risk HPV detection, organized by a provider accredited to ABNT NBR ISO\/IEC 17043:2011.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-275ceee e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"275ceee\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-60cb45e elementor-widget elementor-widget-heading\" data-id=\"60cb45e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Methods<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-80c61d7 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"80c61d7\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-844b1ec elementor-widget elementor-widget-text-editor\" data-id=\"844b1ec\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p align=\"justify\">The EQAP consisted of liquid samples from cervical brushing or cell lysate. Participating laboratories received two samples quarterly to evaluate the accuracy (AC), sensitivity (SE), and specificity (SP), which were thoroughly described. Accuracy trends over time were analyzed using the Mann-Kendall test. Sub-group comparisons included in-vitro diagnostic (IVD) kits versus laboratory-developed tests (LDTs), WHO-recommended kits versus others (non-WHO), and qPCR versus non-qPCR methods. Chi-square tests and odds ratios were employed for statistical comparisons (Table 1).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-38c26bc e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"38c26bc\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-763d217 elementor-widget elementor-widget-image\" data-id=\"763d217\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-1.png\" data-elementor-open-lightbox=\"yes\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MjAxNjUzLCJ1cmwiOiJodHRwczpcL1wvY29udHJvbGxhYi5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL0FydGlnby03LVRhYmVsYS0xLnBuZyJ9\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"446\" height=\"191\" src=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-1.png\" class=\"attachment-large size-large wp-image-201653\" alt=\"Desenho do Estudo\" srcset=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-1.png 446w, https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-1-150x64.png 150w\" sizes=\"(max-width:767px) 446px, 446px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Table 1: Study Design<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-076e119 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"076e119\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-684151b elementor-widget elementor-widget-heading\" data-id=\"684151b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Results<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-6efdca0 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"6efdca0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-25f133c elementor-widget elementor-widget-text-editor\" data-id=\"25f133c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p align=\"justify\">Between 2020 and 2024, 69 labs participated in multiple rounds of the program, with a median of 18 laboratories\/round (range: 5\u201351), generating a total of 692 datasets (Table 2). The laboratories achieved 97.98% (678\/692) AC, 97.79% (620\/634) SE, and 100% (58\/58) SP (Table 3). No significant trends in AC were observed over time (\u03c4=-0.046, p=0.795). IVD kits outperformed LDTs, achieving 98.6% (612\/621) AC compared to 93.0% (66\/71) (OR=5.15, 95% CI 1.68\u201315.83, p=0.009). WHO-recommended kits and non-WHO kits demonstrated comparable AC rates of 98.4% (185\/188) and 98.6% (427\/433), respectively (OR=0.87, 95% CI 0.21\u20133.50, p=1.0). Similarly, qPCR methods showed similar performance to non-qPCR methods, with AC rates of 98.4% (541\/550) versus 96.5% (136\/141) (OR=2.21, 95% CI 0.73\u20136.70, p=0.176) (Table 4).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-eee7cbf e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"eee7cbf\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8e4fed6 elementor-widget elementor-widget-image\" data-id=\"8e4fed6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-2.png\" data-elementor-open-lightbox=\"yes\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MjAxNjU2LCJ1cmwiOiJodHRwczpcL1wvY29udHJvbGxhYi5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL0FydGlnby03LVRhYmVsYS0yLnBuZyJ9\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"425\" height=\"79\" src=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-2.png\" class=\"attachment-large size-large wp-image-201656\" alt=\"Vis\u00e3o geral do participante - Tabela 2\" srcset=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-2.png 425w, https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-2-150x28.png 150w\" sizes=\"(max-width:767px) 425px, 425px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Table 2: Participant Overview<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-de31bd4 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"de31bd4\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-907d42d elementor-widget elementor-widget-image\" data-id=\"907d42d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-3.png\" data-elementor-open-lightbox=\"yes\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MjAxNjYyLCJ1cmwiOiJodHRwczpcL1wvY29udHJvbGxhYi5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL0FydGlnby03LVRhYmVsYS0zLnBuZyJ9\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"417\" height=\"53\" src=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-3.png\" class=\"attachment-large size-large wp-image-201662\" alt=\"Desempenho ao longo do tempo - Tabela 3\" srcset=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-3.png 417w, https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-3-150x19.png 150w\" sizes=\"(max-width:767px) 417px, 417px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Table 3: Performance over time<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-11a744d e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"11a744d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ccdbcbf elementor-widget elementor-widget-image\" data-id=\"ccdbcbf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-4.png\" data-elementor-open-lightbox=\"yes\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6MjAxNjY1LCJ1cmwiOiJodHRwczpcL1wvY29udHJvbGxhYi5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcL0FydGlnby03LVRhYmVsYS00LnBuZyJ9\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"411\" height=\"196\" src=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-4.png\" class=\"attachment-large size-large wp-image-201665\" alt=\"Compara\u00e7\u00f5es de subgrupos - Tabela 4\" srcset=\"https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-4.png 411w, https:\/\/controllab.com\/wp-content\/uploads\/Artigo-7-Tabela-4-150x72.png 150w\" sizes=\"(max-width:767px) 411px, 411px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Table 4: Subgroup comparisons<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-b1f32bb e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"b1f32bb\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1bdcc12 elementor-widget elementor-widget-heading\" data-id=\"1bdcc12\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Conclusions<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-870bf11 e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"870bf11\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1518e42 elementor-widget elementor-widget-text-editor\" data-id=\"1518e42\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p align=\"justify\">The High-risk HPV EQAP demonstrated robust diagnostic performance, with IVD kits significantly outperforming LDTs. Both WHO-recommended and non-WHO kits, as well as qPCR and non-qPCR methods, exhibited comparable AC. These findings highlight the quality of HPV diagnostics in participating laboratories and support preferring IVD tests to address the global burden of HPV-related diseases.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-a8cc8ec e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"a8cc8ec\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7272823 elementor-widget elementor-widget-heading\" data-id=\"7272823\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">References<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"has_ae_slider elementor-element elementor-element-2fffb3a e-flex e-con-boxed ae-bg-gallery-type-default e-con e-parent\" data-id=\"2fffb3a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e71ab69 elementor-widget elementor-widget-text-editor\" data-id=\"e71ab69\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p align=\"justify\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-US\">WHO guideline for screening and treatment of cervical pre-cancer lesions for cervical cancer prevention, second edition: Use of mRNA tests for human papillomavirus (HPV)-World Health Organization, 2021.<\/span><\/span><\/p><p align=\"justify\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-US\">Human papillomavirus (HPV) nucleic acid amplification tests (NAATs) to screen for cervical pre-cancer lesions and prevent cervical cancer: policy brief-World Health Organization, 2022.<\/span><\/span><\/p><p align=\"justify\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-US\">Okada PA, Mitrat S, Rojanawiwat A. External quality assessment program for human papillomaviruses DNA testing in Thailand. Pract Lab Med. 2023 Dec26; 38: e00352. doi: 10.1016\/j.plabm. 2023. e00352. PMID:38292923; PMCID: PMC10825476.<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>G. Barra4, A. Vieira1, L. Vasconcellos2, \u00c1. Pulchinelli3, S. Lima1, E. Santiago1, F. De Aguiar1, S. Lira1, J. Poloni1, V. Biasoli1<\/p>\n","protected":false},"template":"","meta":{"_acf_changed":false},"tags":[2777,2778],"class_list":["post-202147","resumos","type-resumos","status-publish","hentry","tag-desempenho-laboratorial","tag-deteccao-molecular-de-hpv"],"acf":[],"_links":{"self":[{"href":"https:\/\/controllab.com\/en\/wp-json\/wp\/v2\/resumos\/202147","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/controllab.com\/en\/wp-json\/wp\/v2\/resumos"}],"about":[{"href":"https:\/\/controllab.com\/en\/wp-json\/wp\/v2\/types\/resumos"}],"wp:attachment":[{"href":"https:\/\/controllab.com\/en\/wp-json\/wp\/v2\/media?parent=202147"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/controllab.com\/en\/wp-json\/wp\/v2\/tags?post=202147"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}