Efficiency in the analysis of Cavitary Liquids

Efficiency in the analysis of Cavitary Liquids

The study of these liquids is considered an indispensable tool in the delivery of assertive data for the diagnosis

Cavitary liquids analysis is extremely important to provide essential data for the diagnosis, effective therapeutic process and monitoring of pathological conditions, such as complications from chronic liver and heart diseases, as well as tuberculosis and synovitis, among several others.

In recent years, there has been a greater interest in the development of automated analysis of body fluids, mainly due to the limitations of manual cell counting, caused by the lack of experience of the observer or the lack of standardization of the analysis process itself.

Nowadays, several devices have a specific module for the analysis of biological fluids, providing good correlations between manual and automated methods. Specifically for the total counts of nucleated cells (TC-BF) and white blood cells (WBC-BF) in cavitary liquids, according to observations in articles. However, it is worth mentioning that cell differentiation should always be performed using manual light microscopy.

This evolutionary scenario of automation in correlation with manual methods optimizes exam processes, both in terms of time and reproducibility of the analyses. In other words, it allows for a shorter release time and greater reliability of the results.

Cavitary Liquids Processes Control

Faced with the application of manual optical microscopy in the routine, some variables impact the result of the analysis of cavitary liquids, such as: the lack of experience of the observer, the use of inappropriate inputs and the lack of standardization of the process.

Aiming at an adequate performance in the analysis of liquids, each laboratory defines its process flow based on the chosen accreditation standards, in order to obtain a good control and consequently a reliable result.

In these flows, it is recommended that the cellular analysis of cavitary liquids be performed in the Fuchs Rosenthal chamber, according to Prof. Guilherme Dienstmann, Biomedical and Scientific Advisor at Controllab. The performance of the slide for cytomorphological analysis, when in the presence of low cellularity, should be performed after using a cytocentrifuge and with a Romanowski-derived dye.

Additionally, continuous training must be performed with the laboratory professionals, seeking the evolution and optimization of the processes. For an excellent laboratory analysis, the resources provided must be observed, which includes evaluating the need to acquire new inputs and more modern equipment as an investment for this recognition.

For professional updating, scientific article platforms such as Scielo and PubMed can be valuable reference sources. Guidance provided by quality control providers and accreditation standards also complement these sources.

Quality Control in Cavitary Liquids

Quality control must follow the constant evolution of analytical routines. It is possible to identify companies that supply equipment with a specific module for biological liquids together with the commercial control for this module. When the commercial control is produced by a supplier other than the equipment manufacturer, it is possible to detect more sensitive variations, which would not be detected when using the control developed specifically for a given analytical system.

These controls produced by manufacturers other than the analytical system itself are known as third opinion controls. These are independent controls, which generally include inter-laboratory assessment in different systems and reliably reproduce the performance of patient samples, providing an unbiased evaluation. It is also possible that the laboratory itself produces its controls, provided that the chosen accreditation standards are observed.

Applying continuous monitoring (known as Internal Control) and periodic external comparison (known as External Control or Proficiency Testing) builds reliability into analytical routines. The application of Internal Control helps the laboratory to identify random errors that may interfere with the analysis results. When the laboratory participates in the External Control, it is able to identify whether there is an incidence of positive and negative trends occurring in its routine (systematic errors).

Controllab is a laboratory quality control provider with 45 years of experience. It has a qualified technical team advised by specialists in the control areas offered. The statistical analyzes and control of the samples used in the services are rigorously evaluated by these professionals.

Among the controls offered by Controllab, the solutions offered for the Cavitary Liquids routines stand out. The comprehensive program includes both automated and manual methodologies.

Check out Controllab’s Cavitary Liquids programs:

Programs

Tests covered

Available services

MultiparametersBiochemistry

Lactic Acid (Lactate), Uric Acid, Adenosine Deaminase (ADA), Albumin, Amylase, Total Bilirubin, Total Calcium, Chlorides, Cholesterol, Creatinine, Density, Lactic Dehydrogenase (LDH), Ferritin, Alkaline Phosphatase (ALP), GT Gamma, Glucose, Lipase, pH, Potassium, Total Proteins, Sodium, Triglycerides and Urea

Proficiency TestingInternal Control
Immunology

Alpha-1-Acid Glycoprotein, CA 125, CA 15-3, CA 19-9, CEA, Complement C3 and C4

Cell Count

Nucleated Cells and Red Cells, Cell Identification and Cytology

Automated Cell Counting

Nucleated Cells and RBCs

Proficiency TestingInternal Control
Synovial Fluid: Crystals

Crystal Identification

Proficiency Testing

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PRM 0009

Accreditation Cgcre/Inmetro - Certified Reference Material Producer

Controllab has been accredited as a Certified Reference Material Producer (MRC) since 2016 by Inmetro’s General Coordination for Accreditation (CGCRE), under the PMR 0009 number. The assessment of this conformity is carried out according to the requirements established in ABNT NBR standards ISO 17034 and Inmetro, thus ensuring competence for the production of MRCs, according to the Scope of Accreditation.
Cgcre is signatory to the Mutual Recognition Arrangements of the International Laboratory Accreditation Cooperation (ILAC) and Interamerican Accreditation Cooperation (IAAC).

CAL 0214

Accreditation Cgcre/Inmetro – Calibration Laboratory

In December 2002, the Calibration Laboratory was accredited by Inmetro’s General Coordination for Accreditation (CGCRE). With this recognition, it became an integral part of the Brazilian network of accredited laboratories, under nº214. Since then, it is periodically evaluated for the maintenance and/or extension of accreditation.

Cgcre is signatory to the Mutual Recognition Arrangements of the International Laboratory Accreditation Cooperation (ILAC) and Interamerican Accreditation Cooperation (IAAC).

PEP 0003

Accreditation Cgcre/Inmetro - Proficiency Test Provider

In September 2011, Controllab was accredited by Inmetro’s General Coordination for Accreditation (CGCRE) as a proficiency test provider, under nº PEP0003. Initially as part of a pilot project by Inmetro, this accreditation was based on ILAC G13: 2007 and included several clinical and hemotherapy tests. In the periodic evaluations of this accreditation, new segments and tests are included and the evaluation is now conducted according to ABNT NBR ISO/IEC 17043.

This accreditation attests to Controllab’s technical competence to develop and conduct proficiency tests in accordance with international requirements and enables it to be recognized in other countries, in accordance with mutual recognition agreements signed by Brazil in Mercosur, the Americas and Europe.

Cgcre is signatory to the Mutual Recognition Arrangements of the International Laboratory Accreditation Cooperation (ILAC) and Interamerican Accreditation Cooperation (IAAC).